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Spin down the resin as in 1. and SAVE THE SUPERNATANT to assay for uncoupled. Sepharose-4B was activated with cyanogen bromide as described previously. Acetyl 834 companion guide 1.7 to activated Sepharose was performed at pH. 9 at 40 for 16 hr. Gently suspend 1g CNBr-activated Sepharose in 1mM HCl 86µl concentrated.
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NOTE: Carry out all procedures involving antigen in a 4C cold room or on ice. Finally, the CNBr-activated Sepharose is coupled to the.